Edger: How Large Of An Effect Does Moderating The Prior.N Have On Data?
Hi, I'm using the package in R called edgeR to analyze RNA and RIP-seq data. I'm using the moderated gene-wise dispersion method where I choose a prior.n based upon the number of samples I have and how much shrinkage I desire to reduce variance in my data. I've followed the R user guide to achieve gene-wise (tag-wise) dispersion as opposed to common dispersion. I can see that the tag wise dispersion does change for each gene when I change the prior.n value. However I notice that that p-values and logFC values don't change when I change the prior.n. So I'm wondering if I'm doing something wrong or if it's not supposed to change those values? Thanks
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