Base-Call Information Missing In Abi File (Applied Biosystems 3130 Genetic Analyzer)
We need to extent the Base-call information from a .ab1 file generated from the Applied Biosystems 3130 Genetic Analyzer using java program.We able to extract the data from the file using biojava API.
But some of the files we had later missing the Base-call information(decrypted with PBAS and PLOC tags) and we cannot able to proceed without that information.(peaks). But such file contain the raw data channel information(DATA 1-4).
Our knowledge of the sequencing process is less .Can some one explain the reason behind two different ABI files and Is there a way the extract Base-call locations and heights from such file?
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Kanchana Sankalpa •
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