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mm9 genomic annotation UTR, Codon, Promoter

I have a CLIP-seq data and would like to overlay with various genomic features like UTRs, Exons, introns, promoters etc. from mm9 I have looked at the following posts:

but could not figure out how in we can extract such features in one file. Is there any source which may have such GTF/ GFF3 file or any suggestion how I can extract such file with all features from UCSC, biomart or Ensembl

Thanks for any pointer(s)

exons intron 3_prime_utr 5_prime_utr promoter

2 answers

For ChIP-seq I use the bioconductor package ChIPseeker and the function annotatePeak. I see no reason why it would not work also for CLIP-seq data, in essence a set of genomic features just like chip-seq peaks. I really like how easy it is to visualize the comparison of multiple datasets, and you can input your own annotation.

Google suggests something called PIPE-CLIP specifically for CLIP-seq data. I use Homer to annotate ChIP-seq.

Do you use PIPE-CLIP now?

Anyone knows what happened on that program? I got an error on Galaxy site of PIPE-CLIP.

And when I was running it locally, on ubuntu environment I also got error. always can not go through the whole process of that program.

Any suggestions appreciated. Thanks!

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