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Does The Reading Frame Of The Exon Always Start At The First Nucleotide

I have the sequence from an exon. Does the reading frame always start at the first nucleotide of the exon for when I want to translate it to an amino acid?

nucleotide exon
• 3,537 views • link
updated by Ram Ram • written by davidvanijzendoorn • davidvanijzendoorn

1 answer

No, it doesn't. There's no way to know just by looking at an exon what frame is the right frame for translation.

• 236 views • link
updated by Ram Ram • written by swbarnes2 swbarnes2

If you know the length of your 5'UTR you can calculate the frame from the exon sequence, right?

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updated by Ram Ram • written by Phil S. Phil S.

If you know what position that exon sits in the whole transcript, then sure. But you can't know that from the sequence of an exon alone.

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updated by Ram Ram • written by swbarnes2 swbarnes2

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