Merging Illumina Paired End Reads
Dear All,
I have fastq a dataset containing forward and reverse sequences obtained through paired end module of Illumina platform. I am trying to merge these paired end reads. I have a query which I would wish to get cleared before I proceed further. Do I need to get the reverse complement of the reverse sequence dataset in order to carry on with the paired end merging?
I have referred few papers and tutorials on this , but they have not mentioned anything about doing a reverse complement. I am bit confused in this step. Kindly help me out!!!
Responses are highly appreciated!!!
2 answers
duplicate of:
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link
written
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Pierre Lindenbaum
Do you mean overlap merge? Both of the reads are from 5'-->3'.
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link
written
by
Buttonwood •
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