which tool is used for separating forward and reverse from paired end fastq
Hi I have a paired end fastq file downloaded from NCBI, I need to separate these file as a forward and reverse fastq files(like.,R1.fq ,R2.fq). Kindly suggest me some tools for the same.
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ranjini.shakthi •
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how can we know what the input looks like ?
use SRA Toolkit https://www.ncbi.nlm.nih.gov/books/NBK158900/ and use fastq-dump, for example
fastq-dump -I --split-files SRR390728to split paired end reads.But this would not work on local files that haven't been downloaded from SRA, correct?