Oh! automatic link creation for SAM very nice
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to remove ncRNA from raw reads I use Bowtie2 by making a ncRNA index. But after aligning with this index I want to save the reads those are showing no match with this index. how do I do that. I am using fedora 16. Please help me to solve the problem.
The output option section shows:
--un which outputs unpaired unaligned reads to a file --un-conc which outputs paired-end reads that fail to align concordantly into a fileor you take the SAM/BAM output and filter it yourself by the flags
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