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What is the difference between gbk and gbf file?

prokka 1.13.7 is generating gbf file instead of gbk. They look similar. Is there any difference?

annotation prokka
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updated by oschwengers • oschwengers • written by traditionalGuy • traditionalGuy

1 answer

Gbk format is outdated. Gbf replaced it some time (years) ago. From the NCBI Genomes Download (FTP) FAQ:

GenBank flat file format of the genomic sequence(s) in the assembly. This file includes both the genomic sequence and the CONTIG description (for CON records), hence, it replaces both the .gbk .gbs format files that were provided in the old genomes FTP directories.

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written by Arsenal • Arsenal

If gbk format is outdated, why is my Prokka’s version 1.14.5 deleting unwanted file .gbf and changed it to .gbk? Thanks.

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written by neokao • neokao

For some reason NCBI's tbl2asn tool (which Prokka uses to create to GenBank files) writes wrong COORDINATES inference tags. Thus, Prokka fixes this and writes corrected Gbk files -> prokka#L1419

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written by oschwengers • oschwengers

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