PROKKA: File .err - why all my features have inconsistent gene
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6.9 years ago

I am using PROKKA with the following set of arguments:

$ prokka myorganism.fa --cdsrnaolap ON --rfam 1 --rawproduct

While my gbk and gff seems okay, I obtain an error file of 1.2Mb saying that all my features have inconsistent gene locations and "DiscRep_SUB:FEATURE_LOCATION_CONFLICT::Coding region xref gene does not exist".

I though that maybe because my genome is new the IDs are not recognized. Is that right?

PROKKA genome annotation • 2.6k views
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Note: This exact question was also posted on the Prokka GitHub and resolved there: https://github.com/tseemann/prokka/issues/234

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