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Microarray platform probrID to Circ-ID problem
written by ParastooA •I am currently working with circRNA microarray data generated using the Arraystar Human circRNA microarray V2 (GPL21825) platform. However, I am facing a critical issue: …
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obtaining circular RNAs' sequences from circBase
written by aminijavad4 •we can search circBase ID on the circBase website and get information about the circular RNA including DNA position and length of splice. However, it …
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Is third-generation sequencing/long-read-sequencing the only way to identify the full length seque…
written by wangziwei0010 •Thanks for your attention, TLDR: How to get the full length sequence of a novel transcript? Details: 1. There is a novel transcript A I …
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command for to know the count of matching and not matching
written by harry •I have a file in which two-column present one is read and the other is circBase. read circBase hsa_circ_0000160 hsa_circ_0000160 hsa_circ_0000160 hsa_circ_0000160 hsa_circ_0000175 hsa_circ_0000175 hsa_circ_0000175 …
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I want to reverse the fasta sequence
written by harry •I have a large file and I want to reverse the fasta sequence. Please can anyone tell how to do it Thanks in advance
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trimming of fasta file
written by harry •As I have multiple fasta sequences like see below: >read1 CAGGTCTGGCTGGATGAAGGGCACGGCATAGGTCTGACCTGCCAGGGAGTGCTGCATCCTCACAGGAGTCATGGTGCTGCTGAAGATGTCTCCAGAGACCTTCTGCAGGTACTGCAGGGCATCCGCCATCTGCTGGACGGCCTCCTCTCGCCG >read2 CATCTGCGGAGGCTGCCGTGACGTAGGGTATGGGCCTAAATAGGCCATTGTGAGTCATGAGCTTGGTCTGTAGAGGCTGACTGGAGAAAGTTCTGGGCCTGGAGAGGCTGCCGGGAGGTAGGAGTGGTGAGGTCGACTTGAGAAAGTTCAGGGCCTGGAGAGAAGGCTGGGAGGCAGGAGCTGGGTCTAAAGAGGCCATTGTAACGATGGAGCTGTGCCTGTGGAGGCTGTTGTGAGGCAGTAGCCT >read3 TTGAGGTGGGAGGATCGCTTCAGCCTGGAAGGTTGAGGCTGCAGTCAGCTGCGATAGCACTACTACACTCCAGCCTTGGACAACAGAGGGAGACCTTTCGCTGTCACCCCTCTAGAATCCACGTATACGAAAATTCCAAATGTTAGTTGGGCATAGTGGCAAGCACCTGTAGTCTCAGCCACGTGGGAGG These are in different lengths so I want to isolate …
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What's the meaning of the strand+ or strand-?
written by fffuuue •I'm a new explorer in circRNA. I want to get the sequence of a circRNA. But I find my circRNA is different from the other …
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how to get circRNA fasta sequence from CIRI bed
written by fanyucai1 •hello everyone, i use the CIRI to get the bed file of circRNA. But how can i get the circRNA sequence? I try to use …
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circRNA database of TCGA
written by yueli7Hello, TANRIC is a database of exploration lncRNA in cancer. Anyone know is there a database explore of circRNA in cancer which is similar to …
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new gene discovery
written by songmm19900210 •<p>I want to blast my circRNAs which are identified by CIRI software to circRNAs which in circBase database(<a href="http://www.circbase.org/" target="_blank">http://www.circbase.org/</a>). how can i find new …
can please anyone tells me. Thanks in advance