Tools for DNA sequencing saturation analysis
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Thank you for the reply. So do I downsample it to 10%, 20% 50% 80% of the total reads. Then align and variant call again?
Your question was about tools for saturation analysis, the tool I linked will tell you whether sequencing deeper will give you more unique coverage. I have no idea what you are working on, for this you should details to the original question. I assume variant calling.
Apology for not being clear. That exactly what I want to do: to find out if sequencing more will yield more SNPs. Is it correct to use the c_curve function in preseq to do that? Thank you again