How to generate a Consensus sequence from SRA BLAST output from web server?
I searched a gene of interest in a species using SRA BLAST web interface. From graphic summary I know the gene is fully covered with good read support. Now I want to generate a consensus sequence from these reads but I am finding it hard to do so. I will be very very thankful if someone can teach me to do so. The output of aligned reads is in FASTA format. I know it is a naive question to ask, but I am really stuck at this.
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lokdeep17 •
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If you are able to download the aligned fasta reads you should be able to generate a consensus via MEGA (if you need a graphical interface) or via any multiple sequence alignment programs.