Paired sample RNA-seq dataset analysis
Hi everyone,
I need to extract paired (normal vs cancer) RNA-seq data from any dataset. Then find common significant genes between cancer and normal (my gene of interest will fall among significant genes) Next, sort significant genes that most probably their expression is influenced by my gene of interest.
Can you please provide me very detailed workflow and even some personal help. I never done bioinformatics analysis and I truly appreciate anyone who can help me here.
Thanks a lot.
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Survival analysis with RNA-Seq and several cancer subtypes
written by antmantras •
-
correlation analysis of a single variable versus multiple variables
written by pinheirofabiano •
-
How to sort the TCGA dataset based on expression of a panel of genes?
written by dr.goutham •
-
Classify gene ontology (GO) enrichment terms
written by yepeh72919 •
-
Want to get advice on some trans-eQTL result
written by namkungho87 •
-
Differential gene expression based on read counts using DESeq package
written by bioinforesearchquestions
-
How do you download annotations from gramene database
written by p.weru •
-
Identify somatic mutations in cancer exome without a matched pair.
written by rchowdhury •
-
Genome MuSic: Mutation frequency for single gene of interest across all available cancer types (pan…
written by atn3 •
-
Identifying Genes That Express Different Isoforms In Cancer Vs Normal Rna-Seq Data
written by Sahel
If you never did any RNA-seq then please start with https://www.bioconductor.org/packages/devel/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html and the many online resources available via google.
can you please share with me which online dataset I can use to export RNA-seq data. I use following link http://firebrowse.org/?cohort=LUAD&download_dialog=true but the data is not paired it is only lung cancer patients, and there are different formats any idea ?