tell me information about the mutation gene sequence in given below information

column 1:Chromosome
column 2:Position
column 3:Reference allele
column 4:Mutation allele
column 5:Map quality
column 6:Number of Reads mapped in this position by mapping.
column 7:Read frequency: Ratio of reads with mutation allele among all mapped reads in this position.
column 8:Mutation type that annotated by SnpEff (http://snpeff.sourceforge.net/).
column 9:Note1. Information about a neighboring variant in the same gene in the parental strain (DuM).
column 10:Note2. *Mark means that existence of a neighboring variant in the same gene in the mutants.
Column 9 "Note1" and column 10 "Note2" is available when you set a pair of mutant and parental data.