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FFPE samples in NGS

Why do FFPE samples have 3' bias when performing RNA-Seq? Does it also have a GC bias?

#ffpe rna-seq next-gen sequencing

Hello Sujata!

We believe that this post does not fit the main topic of this site.

Please show some effort and read papers that compare RNA-seq data between fresh and FFPE samples.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

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