Thank you so much! And a last one, how do you pull out the genes of interest for comparison and visualization?
What is the recommended approach for quantifying expression of group of genes in specific type of cells?
I want to analyze how a group of genes sharing the same function is expressed in a certain type of cells. I have downloaded 370 gene sequences of genes with needed function (Fasta format files) from a public database. I am planning to download cellular RNA-seq data as well. I would like to quantify expression of these 370 genes in a transcriptome of these cells. What is the recommended workflow for this type of task?
Looking forward to your replies!
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They will just be in an R dataframe. If you aren't familiar with R, some reading will teach you all about them, including how to slice and subset them.
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