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rnaseqcomp for own data withoutref genes

Hi,

Can anyone guide me on how to use rnaseqcomp for own data when we don't have reference genes

https://bioconductor.org/packages/release/bioc/vignettes/rnaseqcomp/inst/doc/rnaseqcomp.pdf

I am comparing kallisto and rsem - quantificationtool comparision.

rnaseqcomp rna-seq quantificaion comparision

RSEM is a widely used tool that produces good results by various benchmarks. On the other hand, a comparison between kallisto and Salmon is pointless because they have been shown in multiple comparisons to produce near identical results. For a recent reference for both these points see https://www.biorxiv.org/content/10.1101/698605v1.abstract

thank you for your comments.

I have seen many articles and found rsem is a better tool, I just want to see the difference in my data. I found a tool rnaseqcomp but it comes with the incomplete manual.

after that, I also want to compare common and specific DE genes of the tools

Lpacther I think I have read almost all of your articles :)

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