This is a test version of Biostars. For the public version, visit https://www.biostars.org.
How to functional annotation bins (the output of MaxBin) of metagenomics

Hi all, I am working with shotgun metagenomics. I got 2 bins from MaxBin (fasta file). Now, I want to annotation the bins. I tried to use RAST but keep having error. I don't know why and also don't know which platforms can help me annotation bins (output of MaxBin). I am a beginner of such analysis. Please help me. THank you in advance.

assembly
• 1,615 views • link
updated by onestop_data onestop_data • written by ttluong2 • ttluong2

1 answer

Hm... very odd. I wonder if RAST is having some problem with the bin contamination. Maybe run CheckM and show how complete/contaminated your bins are.

Also, an alternative for microbial genome annotation is to use prokka.

• 0 views • link
written by onestop_data onestop_data

Log in to answer this question.

More posts like this