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Error in maker (gene prediction)

Hello Everyone,

Does anyone know about below error in maker annotation :

ERROR: Non-unique top level ID While this is technically legal in GFF3, it usually indicates a poorly fomatted GFF3 file (perhaps you tried to merge two GFF3 files without accounting for unique IDs). MAKER will not handle these correctly.

I used fasta file only with one contig. I don't know why I am getting Non-unique ID error.

If anyone know please reply. I am waiting for responses. Thanking you in advance!

Best, Rajesh

software error gene
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written by raj.gzra • raj.gzra

2 answers

You are feeding Maker with a problematic gff file. Could you show us the maker.opts configuration file ?

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written by Juke34 Juke34

Yes, I just solved the issue. And yes, you are right. The problem was with gff file.

Thanks for reply!

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written by raj.gzra • raj.gzra

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