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How to convert paired end data to single end?

Hi,

Can anyone help me out to find a tool or software to convert paired-end data to single-end. I have more than 10k paired end data.

Thanks

rna-seq

1 answer

If your data files are interleaved

@Frag1_Read1
@Frag1_Read2
@Frag2_Read1
@Frag2_Read2

then use reformat.sh from BBMap suite.

reformat.sh in=interleaved_fq.gz out1=R1.fq.gz out2=R2.fq.gz int=t

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