Hello, I am trying to do a de-novo assembly of a turtle genome of about 2.15 gbases with Nanopore Minion reads. I have read about Canu as a good tool for this purpose, but it seems it yields good a good assembly with reads of at least 20x coverage, and the coverage of my reads is only about 2.5x. Is this value too low for de novo assembly with Nanopore reads? What would be a good coverage value?
Thank you everyone