Identification of Long Non coding RNa in De novo
Dear All
I am working on Identification of long non-coding RNAs in scorpion, in this case the genome reference is not available, I have to do blast for to all transcript candidates and I don't find the closest genome. I would appreciate to guide me about the best the best approach.
Best Regards Ayeh
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Could you edit your question with these informations. You could try trinotate : https://github.com/Trinotate/Trinotate.github.io/wiki
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Which type of data do you have ? RNA-Seq ?
Yes, 150 PE and transcriptome assembly process by means of Trinity