Hey,

Here is the way that I did it:

1, in silico predicted gene targets

In order to identify gene targets of micro RNAs (mirs), you can use miRNAtap (Pajak & Simpson, 2015). With this, you can choose different databases:

• DIANA (Vlachos et al., 2012)
• PicTar (Krek et al., 2015)
• TargetScan (Agarwal et al., 2015)
• miRanda (Betel et al., 2008)
• et cetera

Geometric mean score can be used to rank each gene. You can also choose to only include results that appear in 1, 2, 3, or more of te databases.

2, validated

With your output, search for these in the validated MicroRNA-gene Targets section of miRWalk2.0 database (Dweep & Gretz, 2015; Dweep et al., 2011).

Kevin

you can use starBase database http://starbase.sysu.edu.cn/, it provides high-confidence miRNA-target interactions supported by CLIP-seq data.