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Graphical output of Blast. What are grey and black lines?

Hi there!

I was wondering what is the black vertical line separating blue and red bars? How is it different from thin vertical grey line joining red -green bars?

Thank you in advance.enter image description here

blast gaphical output

1 answer

The vertical line is a separation of the single sequence with different alignment score and the horizontal line between green and red is a separation in the same sequence where alignment score is different for different regions.

Hope that helped.

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