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primers for whole metagenome
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15 months ago
Pakistan/Islamabad/Quaid-I-Azam Univers…

Hello All I want to do amplify all the bacterail genomic contents present in environmental samples. What kind of primers I have to use? Primers used for 16s are enough for this procedure? or I have to desgin special type of primers?

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Primers used for 16s are enough for this procedure?

No

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This is not a bioinformatics question. Anyway, buy some multiple displacement amplification kit (just be aware of its shortcomings)..

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This is just shotgun sequencing right? So no use of PCR

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It's a step you perform prior to any sequencing

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11 months ago
Fabio Marroni ♦ 2.2k
Italy

I am sorry for this shameful self-promotion, but you can give a look at the methods section of our recent paper (actually still waiting for reviewers!) in which the library preparation for WGS is explained.

However, I agree with @gb that being shotgun sequencing by definition you must avoid using primers for amplifying any given region.

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