Entering edit mode
5.4 years ago
life99945
▴
20
Hello!
I have forward and reverse illumina myseq 16s rRNA v4-v6 amplicon reads with Pro-mod-341F 5’-CCTAYGGGDBGCWSCAG and Pro-mod-805R 5’-GACTACNVGGGTMTCTAATCC primers used. I want to make sure these primers are already removed. Is there any way to do it?
Thank you.
Thank you very much!
Thank you very much, everything worked out, but I do not understand why in the example they don’t use copyundefined? After all, it greatly affects the reverse read trimming. And how can i count minlength? In example they say that "min and max length were based roughly on 10% smaller and bigger than would be expected after trimming the primers", but how can i expect to get 220 bp after trimming 17bp from 300bp?