Could you tell me why there are multiple peaks in the 16S V3-V4 tapestation data?
Hi researchers.
I would like to know why my results of 1st PCR and 2nd PCR differ from illumina manual. https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf
I ordered the forward and reverse primers listed in the URL from the oligomer design company.
- 16S Amplicon PCR Forward Primer = 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG
- 16S Amplicon PCR Reverse Primer = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC
I think there may be a problem with the primers.
Thank you for your answer.
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