Averaging with technical and biological replicates
Dear all,
I have a data-set consisting of biological and technical replicates and I am interested to use the mean expression of them by initially having the mean of the technical replicates and then the biological replicates before analyzing my data-set using DESEQ2. I wonder if any of you have any suggestion that how can I achieve it within R. I am already aware that there is a collapseReplicates in DESEQ2 but it only deals with technical replicates.
The technical and biological replicated would look like something like below
Day-1_1
Day-1_1
Day-1_2
Day-1_2
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