how can I compare two samples of RNA-seq Generally

I have a general question. what should I do if I want to compare two samples of RNA-seq generally?

thanks in advance

Leila

rna-seq sequence next-gen compare

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updated 8.3 years ago by GouthamAtla

• written 8.3 years ago by lkianmehr

1 answer

Quantify the gene/transcripts --> normalize (TPM/TMM/FPKM/RPKM) and do a Spearman's correlation.

If you dont have annotations, use stringtie to assemble transcript structures.

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written 8.3 years ago by GouthamAtla

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