This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Finding motifs across a genome

Hi community,

I would like to find the genomic coordinates of all the CCGG motifs across my reference genome. The only thought to go around this would be to grep for CCGG across my reference genome and export these sequences in a fasta format. Then align to the same genome and get the coordinates "chromosome" and "position". However, my genome is from a teleost and there are 2 or 3 duplication events so I am not expecting to get all of them aligned uniquely. Also some times a CCGG in a fasta file might be interrupted from one line to the next one so my grep will not be able to get the sequence.

Do you know any other way or some specific software or browser service (UCSC, NCBI, Ensembl) that can do this without aligning?

Regards, Ioannis

genome ccgg

1 answer

fuzznuc from EMBOSS Explorer

Load your reference genome, set your pattern, output the result in tab-delimited format and parse it with unix command or any language you want

It runs like a dream! Thanks a lot Bastien!

Cheers,

Ioannis

Log in to answer this question.