Hi everyone, I’m trying to filter reads based on their mapped length, for example from 1000 bp onwards. Could you suggest something? Would these commands …
Hi. I'am so curious about Padded Alignment. In situation like this, REF: CACGATCA**GACCGATACGTCCGA READ1: CGATCAGAGACCGATA READ2: ATCA*AGACCGATAC READ3: GATCA**GACCG The padded CIGAR are different: READ1: …
Hi. I'am so curious about Padded Alignment. In situation like this, REF: CACGATCA**GACCGATACGTCCGA READ1: CGATCAGAGACCGATA READ2: ATCA*AGACCGATAC READ3: GATCA**GACCG The padded CIGAR are different: READ1: …
<p>Hi, I need to get 5'-end position of each read in <a href='http://samtools.sourceforge.net/SAM1.pdf'>SAM</a> file. For reads aligned in forward direction it's provided in <a href='http://samtools.sourceforge.net/SAM1.pdf'>SAM</a> …
<p>Hi, I would like to calculate the percent-identity from a CIGAR string from a <a href='http://samtools.sourceforge.net/SAM1.pdf'>BAM</a>/SAM file containing alignments. I want to calculate the PID …
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