How to separe eRNA by orientation from with GRO-SEQ data
eRNAs are bi-directionally transcribed.
I was wondering how to separe them from a bam to make distinct bigwig using orientation and strand appartenance.
Hi all, How do I get the strand (transcribed/non-transcribed) information of somatic mutations? I have called the mutations with Mutect2 and annotated them with SnpEff …
<p>I really do not know how a mutation only occur on non-transcribed strand or only on transcribed strand. I think non-transcribed strand and transcribed strand …
Do you want to identify bidirectional transcripts ? or want to create a bigwig that displays transcripts in a strand specific manner ?
Identify bidirectional transcripts, separe them into two different bam, to make 2 bigwigs to display distinctly depending both orientation.