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Cannot match machine name with Machine Type

Hello,

I have a fastq file that says it was run on a machine with a unique ID NB501139 but I don't know how I can match this with a machine type, I assume this is an Illumina machine? I cannot find any Illumina instrument type that would have a name like this? I need this for trimmomatic to know which adapters to look for.

thanks

rna-seq fastq trimmomatic qc
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updated by Devon Ryan Devon Ryan • written by dec986 dec986

There is no correlation between sequencer type and the adapters in your reads. What you are looking for is the type of kit used to make the libraries.

Just use the adapters.fa file included in BBMap suite to trim the data. It contains sequences of adapters from most common library kits. You can use bbduk.sh program included in BBMap as an alternative to trimmomatic.

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written by GenoMax GenoMax

You beat me by 1 minute!

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written by Devon Ryan Devon Ryan

1 answer

That's a NextSeq. Their IDs start with NB (or at least N). Note that the machine type doesn't affect the adapter sequence, just use the standard Illumina (or Nextera, or smallRNA) sequence.

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written by Devon Ryan Devon Ryan

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