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repli-seq analysis pipeline

Hi all,

https://www.illumina.com/science/sequencing-method-explorer/kits-and-arrays/repli-seq.html

Does anybody know a good pipeline to analyze repli-seq data (please see the link above) in order to find origins peaks? I know that most callers are optimized for chip-seq. Do you think MACS is good enough to call this type of peaks?

thank you in advance!

sequencing next-gen chip-seq

Hi! Did you find the way to analyze the data? Im in the same situation!

Thank you very much! One more thing, how is the normalization doing?

As far as I remember I used the normalization from this paper.

But is somehow normalised to the Input or independent of it? Thanks!

It depends on the input BAMs.

what do you mean? can you please be more specific? Thanks!

Were you able to do it? If yes can you share how you did it

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