Lnc RNA,RNA Seq
hi
I have a RNA seq data and planning to do LNC rna analysis.
LNC Rna analysis can be done only on small RNA seq or can be done using Rna seq data?
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Venkatesh •
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sa1987 •
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sa1987 •
2 answers
The l in lncRNAs stands for long, which in this case is >200 bases. Given that, an sRNAseq library prep would get rid of most lncRNAs. You need a ribo-depleted RNAseq dataset (the typical prep uses polyA selection, which will exclude some non-coding RNAs).
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Devon Ryan
You'd have to look at whatever metadata is provided, usually this is mentioned somewhere in there.
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Devon Ryan
This tutorial will give you the analysis workflow for LNC-RNA
Download the tutorial..
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Venkatesh •
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