Dear all,
When I use TBLASTX, I found the result gives an additional column "N", which never appears if using other programs. I really don't know what it means....I even wounder, if this really meaningful? Would any one tell me what it is? Thank you!!
2 answers
used here: https://github.com/LeeBergstrand/NCBI_Tools/blob/281d543937237a80364105b4d14b3671136e06aa/src/objtools/align_format/showdefline.cpp#L663 when eShowSumN is set
eShowSumN is defined in https://github.com/LeeBergstrand/NCBI_Tools/blob/281d543937237a80364105b4d14b3671136e06aa/include/objtools/align_format/showdefline.hpp#L91
eShowSumN = (1 << 4), //Sum_n in blast score structure
so what is this sum_n ? I wonder if it's not the number of hsp (?)
The number in the "N" column indicates how many HSPs were used in computing the statistics for the sequence. If the number is greater than 1, the scores of multiple HSPs were combined to produce the result.
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Is it possible for you to provide a sample output with this column "N" ?
Can you provide your blast cmd? Or did you run on the web?