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how to remove redundant sequences from assembled transcripts

I have assembled the raw reads successfully. Now I want to remove any redundant transcripts from my assembly if there is any. Which too is best suited for it.

Thanks

rna-seq

1 answer

The program will depend on your downstream application but you can use CD-HIT EST to remove redundant sequences at a given sequence identity. It also gives you the longest sequence in the cluster. There are other similar clustering programs too.

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