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Reference based assembly with multiple references

I have a hybrid plant, generated from two distant varieties. I need to assemble its genome with NGS data. We have decided to do a reference based assembly, considering that its repeat content of 50% might be a problem for de novo assembly. Can I do it with multiple reference genomes? That is, with both parental genomes as references?

assembly ngs

1 answer

There are tools that can use multiple references e.g. Multi-CAR or Ragout.

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