why remove adapter just from 3' of the reads
Hello,
I wonder why we just remove adapter from 3' end of reads and not both sides? For example when using Illumina TruSeq™ Small RNA, they use RA5 and RA3 adapter, so why we just trim 3'?
For small RNA, do you trim for quality from both sides of a read if both sides does not have good quality?
Thanks Sara
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jonessara770
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Take a look at the figure at the bottom of this page: http://nextgen.mgh.harvard.edu/IlluminaChemistry.html Unless your inserts are shorter then the length of sequencing you should never see adapters except on the 3'end.
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