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Anti-oligonucleotide design for blocking microRNA, is it even possible?

I want to block the mirna involved in a pathway. So for that i want to design the specific anti oligonucleotide strand so that it will bind the micro rna. please suggest me some procedure to build it computationally.

mirna microrna
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written by bhagyashreemohanty0 • bhagyashreemohanty0

I deleted the other question. On the topic:

  • what makes you sure an anti(sense)-sequence will in fact block miRNA? Any published work doing the same, there is little sense in trying something computationally when the biology doesn't play as we want.
  • how to deliver a single stranded RNA to a cell? (I know about delivering siRNA and double-stranded PCR fragments)?
  • if that worked biological, most likely the antisense sequence of the mature miRNA would work. What makes you think you need a different sequence?
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written by Michael Dondrup Michael Dondrup

You should google for siRNA design or RNAi design, that will give you quite some hits to play with.

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written by WouterDeCoster WouterDeCoster

I think what the OP is looking for is called anti-miRNA antisense inhibitor oligoribonucleotides (AMOs). Single strand antisense oligonucleotides technology predates RNAi by a decade.

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written by Jean-Karim Heriche Jean-Karim Heriche

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