Anti-oligonucleotide design for blocking microRNA, is it even possible?

Question

[bhagyashreemohanty0]

I want to block the mirna involved in a pathway. So for that i want to design the specific anti oligonucleotide strand so that it will bind the micro rna. please suggest me some procedure to build it computationally.

[Michael]

I deleted the other question. On the topic:

• what makes you sure an anti(sense)-sequence will in fact block miRNA? Any published work doing the same, there is little sense in trying something computationally when the biology doesn't play as we want.
• how to deliver a single stranded RNA to a cell? (I know about delivering siRNA and double-stranded PCR fragments)?
• if that worked biological, most likely the antisense sequence of the mature miRNA would work. What makes you think you need a different sequence?

[WouterDeCoster]

You should google for siRNA design or RNAi design, that will give you quite some hits to play with.

[Jean-Karim Heriche]

I think what the OP is looking for is called anti-miRNA antisense inhibitor oligoribonucleotides (AMOs). Single strand antisense oligonucleotides technology predates RNAi by a decade.

[WouterDeCoster]

Oh, perfectly possible :-)

[Michael]

I found a publication describing that: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306207/

Without reading into the details, the problems are more with the lab protocols and in-vivo delivery than with designing an as sequence.

[bhagyashreemohanty0]

thank you for all the replies