Hi,
I have SNPs from RNA-Seq data NGS Analysis.
Which is better for validating cDNA or genomic DNA?
Is there any method available other than sanger for validating these SNPs for multiple samples?
Thanks,
•
written
by
manoharankumar01 •
1 answer
My colleagues (biologists) validate variants on both cDNA (to fit RNA-seq experiment at mRNA level) and genomic DNA (to determine if variant is heterozygous or homozygous) with Sanger sequencing. There also is a good reason to do both: you validate the variant with two independent experiments.
If you have detected many variants (typically >100) and want to validate them, then you may think about working with a dedicated validation platform. It shouldn't cost that much and save a lot of time (which means save money too).
Cheers
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I wouldn't call this a bioinformatics question and this thread can/will get closed because of that, or can you convince me it is bioinformatics?