Filtering out the variants annotated as "FILTER" from vcf file using GATK

Hi,

I am using GATK to filter out variants (specifically SNP's) based on mapping quality

For tagging the variants which failed the MQ(mapping quality) filter, I ran the following commands from GATK

java -Xmx10g -jar /state/partition1/results/GenomeAnalysisTK-3.5/GenomeAnalysisTK.jar  -T VariantFiltration -R reference.fasta -V snp.vcf --filterExpression "MQ < 30.0" --filterName "MQ_snp_filter" -o MQ_filtered_snps.vcf

Later, I verified that it tagged the variants where MQ is less than 30; here is an excerpt from the output vcf file (used AWK to grep few relevant columns):

MQ_snp_filter AC=2;AF=1.00;AN=2;DP=2;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=26.88;QD=17.87;SOR=2.303
MQ_snp_filter AC=2;AF=1.00;AN=2;DP=3;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=23.57;QD=18.76;SOR=1.179
MQ_snp_filter AC=2;AF=1.00;AN=2;DP=4;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=23.18;QD=27.02;SOR=1.609
MQ_snp_filter AC=2;AF=1.00;AN=2;DP=4;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=23.18;QD=35.22;SOR=1.609

Now, I want to remove the tagged variants (remove variants where MQ < 30.0) using GATK. While I went through the help, but could not get to the specific command. Any help would be appreciated.

PS: While I know that there are several ways to filters variants outside GATK, I want to stick to it!

gatk vcf filter snp

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updated 9.6 years ago by Pierre Lindenbaum

• written 9.6 years ago by lakhujanivijay

2 answers

SelectVariants with a JXL expression

java -jar GenomeAnalysisTK.jar \
   -R ref.fasta \
   -T SelectVariants \
   --variant input.vcf \
   -o output.vcf \
  -select 'FILTER != "MQ_snp_filter"'

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written 9.6 years ago by Pierre Lindenbaum

You could do this using SelectVariants:

java -jar GenomeAnalysisTK.jar \
   -R ref.fasta \
   -T SelectVariants \
   --variant input.vcf \
   -o output.vcf \
   -IDs fileKeep \
   -excludeIDs fileExclude

You use either the -IDs or -excludeIDs which is a file containing the variant IDs to respectively include or exclude. You can easily construct that file using some grep/cut/awk manipulations.

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written 9.6 years ago by WouterDeCoster

Thanks WouterDeCoster for the prompt response.

Just to be sure, here ID would be the 3rd column in the vcf file, right?

#CHROM  POS     ID      REF     ALT     QUAL    FILTER  INFO    FORMAT  Sesamum_Indicum.L

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written 9.6 years ago by lakhujanivijay

Yes, indeed. You probably want to make sure those are uniquely chosen/assigned.

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written 9.6 years ago by WouterDeCoster

Uh oh. I just checked the vcf and found that I don't have any ID's in the vcf i.e. the 3rd column just contains a dot for each and every row. Apparently, I missed a step which assigns ID's to the variants? Any clues?

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written 9.6 years ago by lakhujanivijay

A solution is bcftools annotate, SnpSift Annotate or GATK variantannotator. I use the first and the second of those tools for filling in the ID column.

java -Xmx10g -jar SnpSift.jar annotate -id dbsnp.vcf variants.vcf | bcftools annotate --set-id +'%CHROM\_%POS' -  > variantsWithIds.vcf

Using this command the dbsnp identifier is filled in for each position, and for positions which don't have such identifier a combination of the CHROM and POS field is made to form a unique identifier. Note that you can also just use bcftools annotate to set the IDs by position without bothering about dbsnp identifiers.

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written 9.6 years ago by WouterDeCoster