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Differential gene expression between sexes using RNA-Seq

What is an appropriate experimental design appropriate to answer the hypothesis: differential expression of genes in male and female levers

Why are modern tools (EdgeR/DESeq) not the best fit to answer this question?

rna-seq r edger deseq

Definitely looks like one.

modern tools (EdgeR/DESeq) not the best fit to answer this question?

Says who? A source for this statement would help.

I'd be interested in seeing what the "correct" answer to that is too.

I'm more intrigued by this part:

...male and female levers

could it be lovers? lepers? lemurs?

[punchy from sleep deprivation - time for more coffee]

My money is on an organ, liver.

For a moment biostars shows its reddit face. We should really get back on topic. Google suggests "levels" (I googled to look for matches to homework documents posted online)

reddit? Ouch!

I'm now fully caffeinated and back on task. Apologies for the distraction, @Ram. Feel free to delete this part of the thread.

Sorry if that came across as a bit curt - I meant to add a :-) after the "reddit face" part. I just think we should stay a little on topic. I think the "levers" part can give us some clue to if this is a homework question.

But aren't we fully on topic and essentially trying to discover what OP might have on her/his mind? :-)

You could say we are leveraging our joint linguistic skills to dissect the question

Can't argue with that logic.

[Groan for bad wordplay!]

I assumed it was a case of spell-check run amok, but 'livers' and 'levels' make a lot more sense than my guesses.

We need someone thinking outside the box, harold! Don't you dare let your thoughts be bound by something as trivial as "sense"

We all enjoy a good digression :)

Sorry to be a party-pooper! :)

I laughed more than I should. This thread is gold.

Hello hannahzuckerman2!

We believe that this post does not fit the main topic of this site.

This question is vastly opinionated but does provide too little background to be answered.

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