Bio::Tools::Run::RemoteBlast Error: 403 Forbidden

Question

[rplana6]

Hi to everybody;

I am trying to execute a BIOPerl module Bio::Tools::Run::RemoteBlast but I get the error 403 Forbidden. I use a fasta file:

And the parameters I use are:

  my $prog = 'blastn';
  my $db   = 'nt';
  my $e_val= '1e-4';



   my @params = ( '-prog' => $prog,
     '-data' => $db,
     '-expect' => $e_val,
     '-readmethod' => 'SearchIO' );

    my $factory = Bio::Tools::Run::RemoteBlast->new(@params1);

    #change a query parameter

    # This example shows how to change a CGI parameter:
    $Bio::Tools::Run::RemoteBlast::HEADER{'MATRIX_NAME'} = 'BLOSUM50';
    $Bio::Tools::Run::RemoteBlast::HEADER{'GAPCOSTS'} = '15 2';

    # Si no ho posem dóna un warning
    $Bio::Tools::Run::RemoteBlast::MODVERSION = 0;

    #change a retrieval parameter
    $Bio::Tools::Run::RemoteBlast::RETRIEVALHEADER{'DESCRIPTIONS'} = 1000;

    #remove a parameter
    delete $Bio::Tools::Run::RemoteBlast::HEADER{'FILTER'};

    #$v is just to turn on and off the messages
    my $messagesMngm = 1;

I always get the same error, no matter all changes in parameters I do.

Is there anybody who can help me please?

Thanks! Roberto

[Pierre Lindenbaum]

are you working behind a proxy ?

[rplana6]

Hi again,

I've tried it at home and also at work. At home I have a normal Internet connection, at work there is a proxy, yes. Why? Is there a problem? I execute the BLAST via NCBI and it works correctly.

Thanks! Robert

Answer 1

[jyotilakhanimgsu]

NCBI has been shifted from HTTP to HTTPS from September 2016. You have to change protocol in your code from HTTP to HTTPS to run this code. for further detail- visit following link- https://www.ncbi.nlm.nih.gov/home/develop/https-guidance.shtml