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So your data is in fasta format and you want to find pairwise differences with a reference fasta?
Hi I am trying to determine SNP's between a genomic island found in different genomes. The island sequence is about 50kb. Pls suggest me a tool that will help me in the same. Thank you
Thanks for showing interest in my query. To make my post more clear, I have about 25 genome sequences in hand which contain a particular island sequence. I have retrieved the island sequences from each and wish to compare them with an already reported complete sequence of the same island (which would be like a reference) and determine the SNP's (if any). I hope I have clarified.
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So your data is in fasta format and you want to find pairwise differences with a reference fasta?
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Did I understood correctly that you are looking for paralogous sequence variants between multiple copies of a repetitive sequence in multiple reference genomes?