Keep in mind that most of the people here (bioinformaticians) are Linux based, and there is a good reason we stick to Linux for this type of stuff (windows based software are often unstable when dealing with this type of data sets).... BUT!
DNA Star
https://www.dnastar.com/
It's not my favorite because in my opinion it's unstable when dealing with large genomes or a large set of libraries, but it's windows based and allows free trials giving you 2 weeks of free use, and are often available through universities for discounted prices
Geneious
http://www.geneious.com/
Again, trial license is possible and discount for students.
Other than that VMware with Ubuntu and bioinformatics software like @Devon Ryan mentioned is a good option (it's how I play with data on my personal laptop) or you can make a dual boot installation of linux
Another method is getting someone to set up a system called Galaxy, which is essentially an interface for Linux software... it gives windows users a clear GUI with which they can do stuff like assemblies, annotations, etc, problem is that it has to be set up by someone on a Linux backend
And I haven't used this, but there is also a bioinformatics Linux OS called Biolinux
http://environmentalomics.org/bio-linux/
Apparently it's a ready to boot USB OS, so you just plug it in, get your PC to start from USB and you have a complete bioinformatics toolkit at your finger tips :D
FreeandWindows-onlytogether, are deal breakers.What is the expected size of the genome? How big is your dataset?
the probleme is not in the genome size , most softwares that I found require MacOS or Linux system, and I tried with cap3 in the windows system but I have not succeeded
The genome size is part of the problem and influences the answer. Not all software is appropriate for all genome sizes.
You'll be best off running a virtual machine with linux in it on your Windows computer. Then you can use the normal bioinformatics programs.
ok , thank you very much
If you change "Windows" to "web-based", you will have more possible answers and you will still be able to run it on Windows.