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upstream open reading frame gene filteration

Hi Guys,

I have RNA-seq data and want to filter the genes with "upstream open reading frame". do you guys know how to do that? thanks

rna-seq

Hi,

Can you give us more details please ?

Do you want to extract genes with upstream open reading frames (uORF) from Fastq files (reads from sequencing) ? From your annotation file (GFF) ?

so far I have only fastq files. indeed I want to get rid of those genes with upstream open reading frames (uORF) and filter them from my raw data file.

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