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correction for multiple testing

Hi Guys,

after my analysis I have got 3000 genes and I have read counts for all of them in two condition. I want to compare two conditions. do you guys know how to get the p-values for comparison of two conditions (I have 3 columns ). I think I also have to correct for multiple testing? do you guys know how to do that?

Thanks

rna-seq
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updated by andrew.j.skelton73 andrew.j.skelton73 • written by ashkan ashkan

1 answer

See DESeq2

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written by andrew.j.skelton73 andrew.j.skelton73

I am now doing Differential expression analysis. just I want to compare them statistically.

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written by ashkan ashkan

Following the DESeq2 vignette is by far the best way for you to apply the correct statistics. If you're asking what kind of statistical test to use, then DESeq2 implements a Wald test, with Benjamin Hochberg multiple test correction (FDR)

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written by andrew.j.skelton73 andrew.j.skelton73

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