Hi,

I have paired-end sequencing data for 15 cancer and 15 normal samples. I am doing RNA-seq analysis for these samples.

1) While performing RNAseq analysis, is it mandatory to have approximately the same number of reads between the normal and cancer samples?

2) if yes, what is the significance of it?

3) if no, what is the significance of it?

Cancer samples basic stats:

• Min : 21,900,652

• Max : 161,154,015

• Average : 105,993,656

• Stdev : 26,799,534

Normal samples basic stats:

• Min : 87,393,757

• Max : 121,500,267

• Average : 101,632,800

• Stdev : 9,609,422