Entering edit mode
7.6 years ago
kanwarjag
★
1.2k
I have raw counts of miroRNA seq. Do we normalize read count before we apply any t test/ DESEq? I believe we dont need length normlaization
Thanks
So Devon, In other words if I am not using DEseq/EdgeR or Limma we work with read counts without nay further normalization?
To be frank, if you're asking this question then you don't know enough to correctly analyze this type of data without a pre-made R package like DESeq2/edgeR/limma. If you continue this with some method you're coming up with then the results will very likely be wrong.